Dr Valés-Gómez chaired the discussion on different aspects of biomarker discovery based on the detection of EV-associated nucleic acids. Both Dr Otaegui, Dr Peinado and Dr Valés-Gómez presented their results focused on circular RNAs, DNA or mRNA detection, respectively. A lively discussion about methodologies used, limitations and results-driven solutions was held and some ideas and collaborations emerged from the virtual meeting that will benefit the collaborative aspects of the network.